2,7-Dichlorodihydrofluorescein (DCFH2) is a non-fluorescent probe. 2,7-Dichlorodihydrofluorescein produces highly fluorescent 2',7'-dichlorofluorescein (DCF), which can be used for reactive oxygen species measurements.

Physicochemical Properties

Molecular Formula	C20H12O5CL2
Molecular Weight	403.21228
Exact Mass	402.006
CAS #	106070-31-9
PubChem CID	14598431
Appearance	Pink to orange solid powder
Density	1.583g/cm3
Boiling Point	553.7ºC at 760mmHg
Flash Point	288.7ºC
Vapour Pressure	4.26E-13mmHg at 25°C
Index of Refraction	1.717
LogP	5.388
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	2
Heavy Atom Count	27
Complexity	531
Defined Atom Stereocenter Count	0
SMILES	ClC1C(O)=CC2=C(C(C3C=CC=CC=3C(O)=O)C3C(O2)=CC(O)=C(Cl)C=3)C=1
InChi Key	XDFNWJDGWJVGGN-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H12Cl2O5/c21-13-5-11-17(7-15(13)23)27-18-8-16(24)14(22)6-12(18)19(11)9-3-1-2-4-10(9)20(25)26/h1-8,19,23-24H,(H,25,26)
Chemical Name	2-(2,7-dichloro-3,6-dihydroxy-9H-xanthen-9-yl)benzoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

See also: 2',7'-Dichlorofluorescein (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~620.02 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 20.83 mg/mL (51.66 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 208.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (5.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4801 mL	12.4005 mL	24.8010 mL
	5 mM	0.4960 mL	2.4801 mL	4.9602 mL
	10 mM	0.2480 mL	1.2400 mL	2.4801 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.